• 29
    Dec
  • TNF-a but not IL-1a: RESULTS

Effect of PGE1 and D-GalN on serum ALT activity: Thenormal range of ALT activity in rat serum is 18 to 20 U/L (20). In the present study, D-GalN infusion induced a signifi­cant increase 5 and 10 mins after treatment in ALT (29+1.5 and 43±1.2 U/L, respectively) activity compared with the control group (23+0.3 and 27±2.7 U/L, respectively) (P<0.05) (Figure 1). PGE1 did not significantly change ALT activity. Preadministration of PGE1 to D-GalN-treated rats significantly reduced ALT levels to values below those ob­tained in the control group (P<0.05) (Figure 1). Effect of PGE1 and D-GalN on serum TNF-a concentra­tion: The normal range of TNF-a concentration in rat serum is 11 to 18 pg/mL (20). TNF-a release increased significantly 5 to 15 mins after D-GalN infusion compared with the con­trol group (P<0.05) (Figure 2). PGE1 resulted in a significant increase in TNF-a (56±4.7 pg/mL) 15 mins after 0.9% so­dium chloride infusion compared with the control group (33±6.9 pg/mL) (P<0.05) (Figure 2). Preadministration of PGE1 to D-GalN-treated rats caused an additional signifi­cant increase in TNF-a concentration from 1 to 10 mins after D-GalN infusion compared with the D-GalN group (P<0.05) (Figure 2).

Figure 1) Alanine aminotransferase

Figure 1) Alanine aminotransferase (ALT) content in serum from rats treated with D-galactosamine (D-GalN) and/or prostaglandin El (PGEi). ALT was measured at 0, l, 2.5, 5, 10 and 15 mins after D-GalN (200 mg/kg) or 0.9% sodium chloride infusion. PGEi (250 цg/kg) was administered 10 mins before D-GalN or 0.9% sodium chloride infusion. Samples were recovered from the suprahepatic vein. Data are presented as means ± SEM; *P<0.05 compared with the corre¬sponding value of the control group; ^P<0.05 compared with the corresponding value of the D-GalN group

Effect of PGE1 and D-GalN on serum IL-1 a concentration:

D-GalN infusion did not change IL-1 a concentration in serum (Figure 3). PGE1 significantly increased IL-1 a (75±3.9 ng/mL) concentration in serum 15 mins after 0.9% sodium chloride infusion compared with the control group (32±5.7 ng/mL) (P<0.05) (Figure 3). Nevertheless, there were no differences between the D-GalN and PGE1 + D-GalN groups (Figure 3).

Figure 2) Tumour necrosis factor alpha

Figure 2) Tumour necrosis factor alpha (TNF-a) content in serum from rats treated with D-galactosamine (D-GalN) and/or prostaglandin El (PGEi). TNF-a was measured 0, l, 2.5, 5, 10 and 15 mins after D-GalN (200 mg/kg) or 0.9% sodium chloride infusion. PGEl (250 ц-g/kg) was administered l0 mins before D-GalN or 0.9% sodium chloride infusion. Samples were recovered from the suprahepatic vein. Data are presented as means ± SEM. *P<0.05 compared with the corresponding value of control group; ^P<0.05 compared with the corresponding value of D-GalN group

Effect of PGE1 and D-GalN on the concentration of NOx in serum: D-GalN did not essentially modify the concentra­tion of NOx over the time points of the study (Figure 4). Only PGE1 by itself significantly increased the level of NOx 1,10 and 15 mins after treatment compared with the control values (P<0.05) (Figure 4). Nevertheless, preadministration of PGE1 did not modify the concentration of NOx in the PGE1+D-GalN group compared with the D-GalN group (Figure 4). You can afford your pills. Buy online pharmacy generic drugs

Figure 3) Interleukin (IL)1 content in serum

Figure 3) Interleukin (IL)-la content in serum from rats treated with D-galactosamine (D-GalN) and/or prostaglandin El (PGEI). IL-la was measured 0, l, 2.5, 5, l0 and l5 mins after D-GalN (200 mg/kg) or 0.9% sodium chloride infusion. PGEl (250 ц-g/kg) was administered l0 mins before D-GalN or 0.9% sodium chloride infusion. Samples were recovered from the suprahepatic vein. Data are presented as means ± SEM. *P<0.05 compared with the corresponding value of the control group; ^P<0.05 compared with the corresponding value of D-GalN group

Figure 4) The contents of nitric oxide products

Figure 4) The contents of nitric oxide products (NOx) in serum from rats treated with D-galactosamine (D-GalN) and/or prostaglandin El (PGEl). NOx were measured 0, l, 2.5, 5, l0 and l5 mins after D-GalN (200 mg/kg) or 0.9% sodium chloride infusion. PGEl (250 ц-g/kg) was administered l0 mins before D-GalN or 0.9% sodium chloride infusion. Samples were recovered from the suprahepatic vein. Data are presented as means ± SEM. *P<0.05 compared with the corresponding value of the control group; ^P<0.05 compared with the corresponding value of the D-GalN group

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