Hepatitis B Virus (HBV)

Tag: Synthase (Page 3)

Glutathione-Independent Prostaglandin: MATERIALS AND METHODS(4)

Excess MBS was removed by gel filtration at 4°C on a Sephadex (Pharmacia and Upjohn, Kalamazoo, MI) G-25 column equilibrated with 50 mM sodium phosphate, pH 6. Peak fractions of KLH-MBS (1 ml total volume) were coupled with 5 mg peptide dissolved in 1 ml of 250 mM sodium phosphate, pH 7.2. Three female rabbits […]

Glutathione-Independent Prostaglandin: MATERIALS AND METHODS(3)

All samples were centrifuged (1500 X g for 15 min) to remove sperm. The supernatants were centrifuged (15 000 X g for 10 min) and kept at -20°C until use. For in vitro incubation, the samples were incubated as described below. antibiotics levaquin In Vitro Secretion of [35S]Methionine-Cysteine-Labeled Proteins from Tissue Samples and Isolated Tubules […]

Glutathione-Independent Prostaglandin: MATERIALS AND METHODS(2)

Animals and Organ Sampling Eight normal adult rams (Ile de France) and eight normal adult horses (Pony) were used in this study. The efferent ducts of one testis of one animal from each species were ligated with sterile braided silk (close to the extratesticular rete testis) for 30 days in the stallion and 45 days […]

Glutathione-Independent Prostaglandin: MATERIALS AND METHODS(1)

Reagents and Chemicals Dulbecco’s Modified Eagle’s medium without methionine and cysteine (DMEM-), x-ray films (Kodak X-OMAT-XAR5; Eastman Kodak, Rochester, NY), keyhole limpet he-mocyanin (KLH), m-maleimidobenzoic acid A-hydroxysuc-cinimide ester (MBS), orthophenylenediamine dihydrochloride (OPD), 3-([3-cholamidopropyl]dimethylammonio])-1-propanesulfonate, and goat anti-rabbit IgG coupled to horseradish peroxidase were purchased from Sigma Chemical Co. (St. Louis, MO). proventil inhaler

Glutathione-Independent Prostaglandin D2 Synthase in Ram and Stallion Epididymal Fluids: Origin and Regulation

Mammalian spermatozoa acquire their motility and ability to recognize and fertilize oocytes by sequential interactions with proteins present in the epididymal fluid. These epididymal proteins have two main roles: some act directly on spermatozoa to protect and/or mature them, and others are important for the functioning of the organ itself and regulation of its activity. […]