Hepatitis B Virus (HBV)

Tag: Oxovanadium (Page 3)

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(8)

After exposure to these complexes, sperm were washed with 1% TBSA, and the pellets were resuspended in the same medium. The sperm suspension was reacted for 30 min at room temperature with 6 ^g/ml of FITC-conju-gated recombinant human Annexin V (Caltag Laboratories, San Francisco, CA). After two washes in Tyrode’s salt solution, sperm were resuspended […]

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(7)

After incubation, 10 ^g/ml JC-1 (Molecular Probes, Eugene, OR) was added from a stock solution in DMSO (1 mg/ml) to the sperm suspension, which was then incubated for an additional 10 min. At the end of the incubation period, sperm were washed in Tyrode’s salt solution (Sigma), resuspended in 200 ^l of Tyrode’s salt solution, […]

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(6)

Flow Cytometric Assays for Oxovanadium(IV) Complex-Induced Apoptosis We used three independent flow cytometric apoptotic assays to determine oxovanadium(IV)-mediated quantitative changes at the mitochondrial, surface membrane, and sperm nuclear compartments. birth control yasmin

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(5)

Flow Cytometric Quantitation of Sperm Acrosome Reaction In experiments designed to assess the comparative effects of 11 oxovanadium(IV) complexes and a commercial detergent-based spermicide, N-9, on sperm acrosome reaction, motile fractions of sperm (107/ml) prepared from a single donor were incubated in 1 ml of BWW-0.3% BSA in the presence of 100 ^M each of […]

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(4)

The Hamilton Thorne computer calibrations were set at 30 frames at a frame rate of 30 images/ sec. Other settings were as follows: minimum contrast 8; minimum size 6; low-size gate, 1.0; high-size gate, 2.9; low-intensity gate, 0.6; high-intensity gate, 1.4; phase-con-trast illumination; low path velocity at 10 ^m/sec and threshold straightness at 80%; magnification […]

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(3)

A corresponding volume of DMSO (0.25%) was added to the control sperm suspensions. After 3 h of incubation at 37°C, the percentage of motile sperm was evaluated by CASA as described previously. The percentages of motilities were compared with those of sham-treated control suspensions of motile sperm. The spermicidal activity of test compounds was expressed […]

Spermicidal Activity of Oxovanadium: MATERIALS AND METHODS(2)

The choice of these three ancillary ligands (phenanthroline, bipyridyl, and aceto-phenone) was based on the report that the cationic oxovana-dium(IV) complex of phenanthroline is superior to cisplatin (cis-diaminedichloroplatinum([II]) with respect to antitumor activity and on the structural similarity of bipyridyl ring to phenanthroline, as well as on the neutral nature of aceto-phenone complex of oxovanadium(IV). […]