Hepatitis B Virus (HBV)

Tag: Ovary

Gonadotropin-Releasing Hormone Antagonist: DISCUSSION

Several studies performed in rats have demonstrated the in vivo or in vitro antigonadal effects of GnRH analogs. In previous studies, we have demonstrated that GnRH-a treatment produces a failure in the steroidogenic luteal capability and an increase in the apoptotic process in the ovary, eliciting consequently an interference in the follicular recruitment, growth, and […]

Gonadotropin-Releasing Hormone Antagonist: RESULTS

Morphological Studies Prepubertal rats were superovulated with eCG (control group, C) and treated with LA (LA group; 1 ^g rat day during 48 h), or Ant (Ant group; 10 ^g rat day during 48 h), or both (LA + Ant group). When the GnRH-agonist, LA, was injected alone, the ovarian weight decreased by 0.5-fold, whereas […]

Gonadotropin-Releasing Hormone Antagonist: MATERIALS AND METHODS

Hormones and Reagents The GnRH-a leuprolide acetate (Lupron), was a donation from Abbott Laboratories (Buenos Aires, Argentina). The original ampoule (2.8 mg/5 ml) was dissolved in saline solution. SYNTEX S.A (Buenos Aires) generously provided eCG (Novormon). HEPES, SDS, Antide [W-Ac-D-Nal1, D-pCl-Phe2, D-Pal3, Ser4, Nic-Lys5, D-Nic-Lys6, Leu7, Ipr-Lys8, Pro9, D-Ala10NH2; Nal-Lys antagonist] was purchased from Sigma […]

Gonadotropin-Releasing Hormone Antagonist: INTRODUCTION

In the mammalian ovary, only a small fraction of oocytes ovulate during reproductive life, whereas the majority of ovarian follicles undergo atresia by a hormonally regulated apoptotic mechanism. Gonadotropin-releasing hormone (GnRH) and its analogs have been widely used to prevent the spontaneous LH surge in assisted reproduction techniques. Suppression of gonadotropin secretion can be achieved […]

Tight Junctions in Human Ovarian Surface Epithelium: DISCUSSION

The ovarian surface epithelium (OSE) is a modified peritoneal mesothelium. It is composed of a single-layered sheet of flat to cuboidal cells that rest on a basal lamina and express microvillus toward the peritoneal cavity. The OSE is distinguished from extraovarian mesothelium by its capacity of steroid synthesis and expression of keratin and mucin. Epithelial-derived […]

Tight Junctions in Human Ovarian Surface Epithelium: RESULTS

Tight Junction Proteins in Human Ovarian Biopsies By immunofluorescence microscopic analysis, we could determine that the main localization of the TJ-associated protein ZO-1 and the integral membrane proteins occludin and claudin-1 was at the cell borders of normal OSE (Fig. 1, A through C). The underlying stroma were without staining, indicated by stars. TJ protein […]

Tight Junctions in Human Ovarian Surface Epithelium: MATERIAL AND METHODS

Patient Material OSE cells and tissue biopsies from normal ovaries were obtained from women operated on for benign nonovarian diseases at the Gynecology Unit, Sahlgrenska University Hospital, Goteborg, Sweden. OSE cells for culture were obtained from a total of 32 women. Normal ovarian tissue biopsies were collected from a total of nine women. A description […]

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