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  • Glutathione-Independent Prostaglandin: DISCUSSION(2)


The secretory activity was highly regionalized and was localized only in the initial segment (regions 0-1) of the ram epididymis and regions 0-2 (proximal and distal caput) of the stallion epididymis. Expression of mRNA for PGDS in ep-ididymal tissues was detected only in the caput epididymal region where the protein was secreted. The secretion of PGDS in both the ram and horse was among the highest in this region (around 20%), and PGDS was a major protein of the epididymis, accounting for nearly 8% of all proteins secreted by the epididymis. In both species, the highest amount of PGDS was found in the fluid of the anterior region (around 10%). This protein could be considered as a good marker of the secretory activity of the anterior region, as also proposed for GPX. buy flovent inhaler

The amount of PGDS progressively decreased during epididymal transit and reached a value 5-fold lower in the caudal fluid compared to the caput. This decrease could be linked in part to resorption of this protein by the epithelium, as has been described for other proteins such as clusterin and GPX. The decrease could also be the result of partial degradation of the molecule in view of the progressive loss of its mass. Furthermore, the pI of PGDS isoforms in the ram became more basic in the cauda than in the caput. These modifications are probably linked to changes in the carbohydrate part of this glycoprotein that possesses two potential A-glycosylation sites.

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