Hepatitis B Virus (HBV)

Expression of 11 p-Hydroxysteroid Dehydrogenase: RESULTS(1)

RESULTS(1)

Ovarian Expression of 11/5HSD1, 11/5HSD2, GR, and MR mRNAs in immature Gonadotropin-Treated Rats (Fig. 1)

All mRNAs were detected in the ovary, although signal intensity varied between mRNA species and stage of follicular development. The expression of 11pHSD1 was very low in group C but dramatically up-regulated in groups P (783 ± 133% of the control value, mean ± SEM; p < 0.01) and H (2835 ± 878%; p < 0.01). The expression of 11 pHSD2 remained unchanged in group P (111 ± 7%) and was decreased in group H (34 ± 6%; p < 0.01). Expression of GR was unchanged in both groups P (99 ± 14%) and H (107 ± 18%), while expression of MR was down-regulated in groups P (51 ± 15%; p < 0.05) and H (25 ± 4%; p < 0.01). birth control yasmin

Ovarian Expression of 11pHSD1 and 11pHSD2 mRNAs during Luteinization (Fig. 2)

Expression of 11pHSD1 increased steadily after hCG treatment, reaching a maximum of 2000-3000% of the control value at 9-24 h and falling to 600% at 120 h. Conversely, expression of 11pHSD2 decreased after hCG, reaching a nadir of 40-30% of the control value at 6-12 h and recovering to 70% at 24-120 h.

Expression of 11pHSD1, 11pHSD2, GR, and MR mRNAs in Isolated Granulosa Cells, Residual Ovary and CL (Fig. 3)

All four mRNAs were detected in isolated granulosa cells, residual ovary, and CL. Levels of both 11pHSD mRNAs were 1.5-2 times higher in the residual ovary than in the granulosa cells, while equivalent levels of GR and MR mRNAs were detected in both tissues. Relatively high but variable levels of these mRNAs were also detected in CL.

Expression of 11 (3HSD1 was very low in granulosa cells from group P but was clearly detectable in residual ovary. After hCG treatment, the expression of 11pHSD1 increased in both granulosa cells and residual ovary. The expression of 11 pHSD2 and MR in granulosa cells and residual ovary responded to gonadotropin treatment in the same way and to a similar extent. The expression of GR was not measurably altered by any treatment, and similar levels of GR mRNA were detected in isolated granulosa cells and residual ovary.
Fig1Expression of
FIG. 1. Effect of gonadotropins on ovarian 1ipHSD1, 11PHSD2, GR, and MR mRNA expression in immature rats. Top) Autoradiogram of ri-bonuclease protection assay showing protected fragments of 11 PHSD1, 11 PHSD2, GR, and MR mRNAs and 18S rRNA (lower panels). Approximately 20 ^g of total RNA was analyzed for 11 0HSD1, GR, tRNA, kidney (Kd), and liver (Lv) and 40 ^g for 11 PHSD2 and MR. Films were exposed for 4 days (11 PHSD1 and Gr), 9 days (11 PHSD2 and MR), or 1 day (18S rRNA). Bottom) Quantitative analysis of gene expression. Data were normalized using 18S rRNA and expressed as a percentage of the control values. Results were expressed as mean ± SEM of three separate trials. * and **, Significantly different from the control values; p < 0.05 and p < 0.01, respectively (ANOVA with Student’s f-test).

Fig2Expression of
FIG. 2. Ovarian expression of 11 pHSD1 and 11 pHSD2 mRNAs during gonadotropin-stimulated luteinization in immature rats. Top) Autoradiogram of ribonuclease protection assay showing protected fragments of 11pHSD1, 11 pHSD2, and 18S rRNA (lower panels). Approximately 20 ^g of total RNA was analyzed for 11 pHSD1 and 40 ^g for 11 pHSD2. Films were exposed for 4 days (11 pHSD1), 7 days (11 pHSD2), or 1 day (18S rRNA). Kd, kidney; Lv, liver. Bottom) Quantitative analysis of gene expression. Data were normalized using 18S rRNA and expressed as a percentage of the control values.

Fig3Expression of
FIG. 3. Expression of 11 pHSD1, 11 pHSD2, GR, and MR mRNA in isolated granulosa cells, residual ovary, and CL during gonadotropin-stimulated follicular maturation and luteinization in immature rats. Top) Autoradiogram of ribonuclease protection assay showing protected fragments of 11 pHSD1, 11 pHSD2, GR, and MR mRNAs and 18S rRNA (lower panels). Approximately 20 ^g of total RNA was analyzed for 11 pHSD1, 11pHSD2, and GR; 40 ^g for MR. Films were exposed for 6 days (11 pHSD1 and GR), 14 days (11 pHSD2 and MR), or 1 day (18S rRNA). Bottom) Quantitative analysis of 11 pHSD1, 11pHSD2, GR, and MR mRNA gene expression. Data were normalized using 18S rRNA and expressed as a percentage of the control values. Results were expressed as mean ± SD of two separate trials.

Category: Ovary

Tags: Hydroxysteroid Dehydrogenase, Ovary, Receptor Genes

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