• 2
    Feb
  • Comparative Studies on the In Vitro: DISCUSSION(1)

This is the first report that describes in vitro deciduali-zation in a nonhuman primate. We have demonstrated that baboon stromal cells require dbcAMP in addition to hormones to produce high levels of IGFBP-1.

Evidence presented in this study suggests that the processes of decidualization in the human and baboon involve similar mechanisms. Both human and baboon stromal cells expressed aSMA in culture and, in response to hormones and dbcAMP, high levels of IGFBP-1. Furthermore, stromal cells from the human and baboon were responsive to shortterm dbcAMP treatment after pretreatment with hormones. Within each species, decidualized and nondecidualized cells did not differ in their expression of PKA regulatory subunit isoforms. Thus, these two species appear to possess similar metabolic pathways necessary for decidualization, but the process of decidualization differs in its sensitivity or degree of responsiveness to external stimuli. Human de-cidualizing stromal cells generally produced higher levelsĀ of prolactin and IGFBP-1 than baboon cells and were more responsive to dbcAMP.

This is the first report that describes in vitro deciduali-zation in a nonhuman primate. We have demonstrated that baboon stromal cells require dbcAMP in addition to hormones to produce high levels of IGFBP-1. ventolin inhalers

Evidence presented in this study suggests that the processes of decidualization in the human and baboon involve similar mechanisms. Both human and baboon stromal cells expressed aSMA in culture and, in response to hormones and dbcAMP, high levels of IGFBP-1. Furthermore, stromal cells from the human and baboon were responsive to shortterm dbcAMP treatment after pretreatment with hormones. Within each species, decidualized and nondecidualized cells did not differ in their expression of PKA regulatory subunit isoforms. Thus, these two species appear to possess similar metabolic pathways necessary for decidualization, but the process of decidualization differs in its sensitivity or degree of responsiveness to external stimuli. Human de-cidualizing stromal cells generally produced higher levelsĀ of prolactin and IGFBP-1 than baboon cells and were more responsive to dbcAMP.

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