Hepatitis B Virus (HBV)

Category: Pregnancy

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: DISCUSSION(3)

In the present study, marked differences in UTMP mRNA expression were apparent in sGE between Days 17 and 50 of pregnancy, with higher levels of UTMP mRNA in the upper sGE compared to the lower sGE near the myometrium. This dichotomy in expression may be related to the fact that deeper glands of the intercaruncular […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: DISCUSSION(2)

In the present study, overall changes in steady-state levels of intercaruncular endometrial UTMP mRNA during pregnancy closely paralleled changes in PL production by the placenta and release of newly synthesized proteins, including UTMP, by intercaruncular endometrial explants from pregnant ewes. Spencer et al. reported that intrauterine administration of recombinant oPL increased proliferation of sGE and […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: DISCUSSION(1)

Expression of PRL-R by uterine endometrium has been reported for the cow, human, pig, rat , rabbit, and ewe. As in the cyclic human uterus, pRL-R expression was expressed specifically in the endometrial GE. Results from this study and others extend those of Cassy et al. in sheep and Jones et al. in the human by […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: RESULTS(3)

ImmunohistochemicalAnalysis of PRL-R Protein in the Ovine Uterus The location of PRL-R protein in the cyclic and pregnant ovine uteri from study 1 was assessed using immunofluorescence antibody analysis of frozen uterine sections (Fig. 5). Immunoreactive PRL-R protein was present exclusively in the apical aspect of the endometrial sGE of both cyclic and pregnant uteri. […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: RESULTS(2)

As shown in Figure 2B, both long (310 bp) and short (207 bp) forms of the ovine PRL-R mRNA were present in the intercaruncular endometrium of Day 20 to 120 pregnant ewes from study 2. Two specific PCR products (310 bp and 350 bp) were consistently detected using the long PRL-R mRNA primers. Sequence analysis […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: RESULTS(1)

Steady-State Levels of Endometrial PRL-R mRNA Steady-state levels of PRL-R mRNA in the endometrium were affected by day of the estrous cycle and pregnancy (Fig. 1). In cyclic ewes, endometrial PRL-R mRNA levels were affected (P < 0.09, cubic) by day, but changes were not dramatic (Fig. 1A). Between Days 11 and 19 of pregnancy […]

Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium: MATERIALS AND METHODS(5)

Immunocytochemical Analysis Frozen sections (4-8 ^m) of uterine tissues embedded in OCT compound in study 1 were cut with a cryotome (Lipshaw Manufacturing, Detroit, MI) and mounted on Superfrost/Plus microscope slides (Fisher Scientific). Concep-tuses were present in the uterine lumen of sections from Day 19 pregnant ewes, as these uteri were not flushed prior to […]

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