Hepatitis B Virus (HBV)

Category: Ovary (Page 2)

Expression of 11 p-Hydroxysteroid Dehydrogenase: MATERIALS AND METHODS(3)

Each PCR cycle consisted of 45-sec denaturing at 94°C, 45-sec annealing at 60°C, and 2-min extension at 72°C with the final extension for 10 min. Resultant PCR products were cloned using pCR-Script Amp SK(+) Cloning Kit (Stratagene Cloning Systems) and sequenced to verify the authenticity of the products. The 18S rRNA was synthesized from cDNA […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: MATERIALS AND METHODS(2)

RNA Preparation Ovaries were homogenized in ice-cold 4 M guanidium thiocyanate solution containing 25 mM sodium citrate, 0.5% (w:v) sarcosyl, and 0.1 M (3-mercaptoethanol (all from Sigma). Total RNA was extracted with phenol-chlo-roform. buy yasmin online 32P-Labeled Probes The 11(3HSD1, 11(3HSD2, GR, and MR RNA probes were synthesized from DNAs generated by reverse tran-scription-polymerase chain […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: MATERIALS AND METHODS(1)

Animals and Treatments Immature female Wistar rats (21-25 days old; Charles River UK, Margate, UK) were kept in a temperature-controlled room on a 12L:12D cycle and fed rat chow and water ad libitum. Animals were assigned to three groups and treated (subcutaneous injection in PBS vehicle) as follows before being killed: 1) eCG (Sigma Chemical […]

Expression of 11 p-Hydroxysteroid Dehydrogenase, Glucocorticoid Receptor, and Mineralocorticoid Receptor Genes in Rat Ovary

In glucocorticoid (G) and mineralocorticoid (M) target organs, access of these steroids to their receptors (Gr and MR) is regulated by two 11p-hydroxysteroid dehydrogenase (11pHSD) isoforms: NADP+-dependent bidirectional 11pHSD type 1 (11pHSD1) with predominant reductase activity and low binding affinity for cortisol or corticosterone, and NAD+-dependent dehydrogenase type 2 (11pHSD2) with high binding affinity for […]