Hepatitis B Virus (HBV)

Category: Ovary

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(6)

The finding that 11 pHSD genes are gonadotropically regulated in rat ovarian granulosa cells is consistent with a shift in G metabolism from inactivation (due to oxidation by 11pHSD2) to activation (reduction by 11pHSD1) during hCG-induced granulosa cell luteinization. Together with the elevated circulating level of adrenal-derived corticosterone that occurs around the time of the […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(5)

We find that in addition to GR, the rat ovary expresses MR mRNA. Coexpression of MR and 11 (3HSD2 is a feature of M target organs such as kidney and colon. Coordinate regulation of both genes in the ovary suggests that it may also be an M target organ. However, there is no known physiological […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(4)

The presence of GR mRNA confirms isolated granulosa cells and CL as G target tissues. Glucocorticoids or synthetic G, dexamethasone, has been shown to increase FSH-stimulated progesterone (P) production in cultured immature granulosa cells. This is at least partially due to a selective increase in 3 pHSD activity and a decrease in 20aHSD activity. Glucocorticoids […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(3)

This is presumably due to the difference in LH responsiveness of thecal-interstitial cells and granulosa cells, since the latter only express LH receptor after adequate exposure to FSH. The inability to remove all granulosa cells from the ovary using the puncture technique also clouds the interpretation of the ‘‘residual ovary’’ Mr mRNA data. It is […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(2)

11pHSD1 has previously been localized by immunocyto-chemistry and in situ hybridization to oocytes and CL, but not granulosa cells or thecal-interstitial cells on the morning of proestrus, i.e., shortly before onset of the LH surge. It was also demonstrated in that study that NAD-dependent 11pHSD activity (i.e., 11pHSD2) is present in the ovary of the […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: DISCUSSION(1)

This study provides direct evidence that 11 (3HSD genes are gonadotropically regulated in the mammalian ovary. Previously we showed that luteinized granulosa cells from the ovaries of in vitro fertilization patients, collected 36 h after ovulation induction with hCG, express increased levels of 11pHSD1 mRNA but reduced levels of 11pHSD2 mRNA as compared with granulosa […]

Expression of 11 p-Hydroxysteroid Dehydrogenase: RESULTS(1)

Ovarian Expression of 11/5HSD1, 11/5HSD2, GR, and MR mRNAs in immature Gonadotropin-Treated Rats (Fig. 1) All mRNAs were detected in the ovary, although signal intensity varied between mRNA species and stage of follicular development. The expression of 11pHSD1 was very low in group C but dramatically up-regulated in groups P (783 ± 133% of the control […]

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