Hepatitis B Virus (HBV)

Category: Oocyte

Stimulation of Xenopus laevis Oocyte Maturation: DISCUSSION

Me-p-CD has been used extensively as a pharmacological tool to deplete cellular cholesterol, disrupt cholesterolrich membrane domains, and investigate coincident changes in signaling phenomena and cellular responses. In cultured mammalian cells, 5-100 mM cyclodextrin has been used, and longer exposure to high concentrations is sometimes toxic to cells. Therefore, the toxic response observed in some […]

Stimulation of Xenopus laevis Oocyte Maturation: RESULTS

To test the effect of cholesterol depletion on the time course of progesterone-induced GVBD, groups of 25-30 oocytes were incubated in increasing concentrations of Me-p-CD in Ringers-HCO3 at room temperature for 4 h, washed, and incubated with 1.5 |xM progesterone. Treatment of oocytes with concentrations of Me-p-CD <5 mM had no apparent effect on the […]

Stimulation of Xenopus laevis Oocyte Maturation: MATERIALS AND METHODS

Experimental Animals and Oocyte Isolation Mature Xenopus laevis females (Xenopus I, Dexter, MI) were housed in aquatic tanks in a temperature controlled room at 16°C, fed ground beef twice weekly, and maintained on daily cycles of 14L:10D. Frogs were primed by injection of 35 IU of eCG (PmSg; Calbiochem, La Jolla, CA) into the dorsal […]

Stimulation of Xenopus laevis Oocyte Maturation: INTRODUCTION

Accumulating evidence supports a role for low-density membrane (LDM) microdomains (rafts and caveolae) in cell signaling and in transmembrane and transcellular processing (for reviews, see ). Cholesterol-enriched LDM containing immunodetectable caveolin-like protein has been recovered from Xenopus laevis oocytes. Comparison of past observations in the oocyte system with more recent evidence in other cells suggests […]