Hepatitis B Virus (HBV)

Category: Isoforms

Null Allele Encodes Functional HLA-G Isoforms: DISCUSSION

After our study of the tolerogeneic role of HLA-G in maternal-fetal tolerance, we designed experiments to identify the structural and functional characteristics of proteins generated by the HLA-G*0105N allele. First, we used site-directed mutagenesis to construct the HLA-G*0105Nfrom the nucleotide sequence of HLA-G*010102 and transfected it into the M8 human cell line. Then we characterized […]

Null Allele Encodes Functional HLA-G Isoforms: RESULTS

Construction of HLA-G*0105N Null Allele Genomic DNA To study proteins encoded by the HLA-G null allele, we generated a DNA sequence identical to HLA-G*0105N in the pcDNA 3.1 expression vector. In vitro site-directed mutagenesis of HLA-G*010102 genomic DNA allowed us to delete the cytosine at position 815 from ATG (exon 1), yielding the genomic dNa […]

Null Allele Encodes Functional HLA-G Isoforms: MATERIALS AND METHODS

in Vitro Site-Directed Mutagenesis In vitro site-directed mutagenesis was carried out using the Stratagene QuickChange XL site-directed mutagenesis kit, which allows site-specific mutation in double-stranded plasmids. The plasmid DNA template (50 ng) HLA-G*010102-pcDNA3.1, isolated from the dam+ JM109 Escherichia coli strain, was replicated by PfuTurbo DNA polymerase (2.5 U) using two synthetic primers, G.Null S […]

Null Allele Encodes Functional HLA-G Isoforms: INTRODUCTION

Protection of the semiallogenic fetus against maternal immune recognition and attack has been attributed principally to the high and almost unique expression of human leukocyte antigen-G (HLA-G) on trophoblast cells at the fetal-maternal interface. The nonclassical HLA-G gene is characterized by alternative splicing that yields seven proteins, four membrane bound (HLA-G1 through -G4) and three […]