Hepatitis B Virus (HBV)

Category: Fluids

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: DISCUSSION(4)

Because enzyme activity of PGDS in the epididymal fluid is unlikely, we investigated its function as a putative androgen and RA transporter. RA was a good candidate, because 1) it is present in the epididymis, 2) it is important for maintenance of the epididymal epithelium, and 3) Tanaka et al. previously demonstrated that recombinant human […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: DISCUSSION(3)

Taken together, these results strongly suggest that the enzymatic activity of PGDS is very low (or even absent) in the epididymal lumen, with our experimental conditions being close to in vivo conditions. Previous studies demonstrating the existence of enzymatic activity of epididymal PGDS were performed in drastic conditions (pH 9) that seem incompatible with the […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: DISCUSSION(2)

Several putative sites of glycosylation (N and O) were observed in the protein sequences for both species. We have previously described PGDS isoforms using 2D electrophoresis in the epididymal fluid of rams and stallions. The difference of 8-10 kDa between the theoretical and the apparent molecular mass of PGDS and the presence of at least […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: DISCUSSION(1)

In this study, we investigated the functional and biochemical characteristics of PGDS, a major protein secreted in the mammal epididymis present in the milieu surrounding the spermatozoa throughout the male genital tract. To evaluate the function of PGDS in male reproduction, we first analyzed the variations in the presence of PGDS in the semen and […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: RESULTS(5)

To summarize our investigation of this pathway, PGD2 production in the epididymis under our experimental conditions was quantitatively irrespective of the presence of PGDS in the lumen. These results strongly suggest that luminal PGDS is not involved in the enzyme production of PGD2. These results were corroborated by the absence of PGD2 production when PGH2 […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: RESULTS(4)

Metabolic PG pathway in the epididymis. The presence of COX1 in the ram epididymis indicated that the PG pathway is functional in this organ. To investigate this pathway, we used an in vitro incubation technique with closed-end epididymal tubules as previously described in the presence of [3H]arachidonic acid. The radioactive PGs produced in the luminal […]

Mammalian Lipocalin-Type Prostaglandin D2 Synthase in the Fluids of the Male Genital Tract: RESULTS(3)

Metabolic PG Pathway in the Epididymis To investigate the putative enzymatic function of PGDS, we performed an overall study of the metabolic PG pathway in the epididymis. The aim of this approach was to determine if this pathway is functional in the epididymis by detecting the cyclooxygenases (COX1 and COX2) in the ram epididymis, analyzing […]

Tags