Hepatitis B Virus (HBV)

Category: Epididymal

Glutathione-Independent Prostaglandin: DISCUSSION(6)

Another potential source of PGH2 may be the spermatozoa themselves, since cyclooxygenases have also been localized in the male gamete. The hypothesis of a sperm PGH2 origin is reinforced by the fact that arachidonic acid (the substrate of COX activity) can be released from sperm membranes by phospholipase A2 during the loss of phospholipids as […]

Glutathione-Independent Prostaglandin: DISCUSSION(5)

The role of PGDS in all the fluids of the genital tract is uncertain. No information is available in vivo about enzyme activity of the secreted luminal PGDS. However, PGD2 can be formed in vitro by glutathione-independent PGDS in rat epididymal tissue homogenates, and thus it is probable that PGDS is potentially active in the […]

Glutathione-Independent Prostaglandin: DISCUSSION(4)

The origin of PGDS in the seminal plasma of the ram was the epididymis, since no PGDS mRNA was detected in the seminal vesicle tissue and the protein was not detectable in seminal vesicle secretions or in the seminal plasma of vas deferens-ligated rams (data not shown). Buy Advair Diskus Online The regulation of expression […]

Glutathione-Independent Prostaglandin: DISCUSSION(3)

The presence of PGDS in ram and stallion epididymal fluids was related not only to epididymal activity but also to testicular activity, as it was detected in the RTF. This protein has also been found in the RTF of the bull. However, testicular PGDS has more acidic isoforms than the epididymal protein. As only one […]

Glutathione-Independent Prostaglandin: DISCUSSION(2)

The secretory activity was highly regionalized and was localized only in the initial segment (regions 0-1) of the ram epididymis and regions 0-2 (proximal and distal caput) of the stallion epididymis. Expression of mRNA for PGDS in ep-ididymal tissues was detected only in the caput epididymal region where the protein was secreted. The secretion of […]

Glutathione-Independent Prostaglandin: DISCUSSION(1)

In the present study, a major secreted protein was identified in the testicular, epididymal, and seminal fluids of rams and stallions as the glutathione-independent lipocalin-type PGDS (prostaglandin-H2 D-isomerase; (5Z,13E)-(15S)-9a,11 a-epidioxy-15-hydroxyprosta-5,13-dienoate-D-isomerase, EC 5.3.99.2). We identified this compound by microsequencing the protein and by using polyclonal antisera obtained from a peptide deduced from the cDNA sequence of […]

Glutathione-Independent Prostaglandin: RESULTS(4)

A 0.8-kilobase mRNA was detected only in the proximal caput epididymidis of both the ram (regions 0-1) (Fig. 4C) and the stallion (regions 0-2) (Fig. 7C). Testicular PGDS transcripts were found in stallions (not shown), but we were not able to detect signals in any rams. PGDS mRNA could be detected in the efferent ducts […]

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