Hepatitis B Virus (HBV)

Category: Embryo

Effect of 1,2-Propanediol Versus 1,2-Ethanediol: DISCUSSION

This is the first examination of the impact of permeating cryoprotectant (PrOH or EG) on the immature cat oocyte and its ability to undergo subsequent maturation, spindle formation, fertilization, and development in vitro. Although high proportions of oocytes resumed meiotic maturation after CPA exposure, progression past the MI stage generally was impaired at high CPA […]

Effect of 1,2-Propanediol Versus 1,2-Ethanediol: RESULTS

Effect of CPA Exposure on In Vitro Nuclear Maturation ofOocytes In experiment 1, percentage of oocytes remaining at the germinal vesicle stage (range of means, 8.8%-15.2%) was not affected (P > 0.05) by the CPA type, concentration, or exposure temperature and was not different (P > 0.05) from controls (6.5 ± 7.7% at 25°C; 12.9 […]

Effect of 1,2-Propanediol Versus 1,2-Ethanediol: MATERIALS AND METHODS

Immature Oocyte Collection, Cryoprotectant Exposure, and In Vitro Maturation Ovaries from adult domestic cats were collected during the breeding season (December-June) from local veterinary clinics and transported to the laboratory within 6 h of ovariectomy in PBS at 4°C. Immature oocytes were recovered by slicing the ovaries with a scalpel blade in H-MEM (Hepes-buffered Minimum […]

Effect of 1,2-Propanediol Versus 1,2-Ethanediol: INTRODUCTION

Oocyte cryopreservation could be a viable tool for preserving the female genome to assist in genetically managing rare individuals and small populations. However, survival and quality of frozen-thawed oocytes are poor compared to fresh counterparts for a host of mammalian species, including the hamster, rabbit, pig, mouse, cow, and human. Oocyte survival and quality in […]