• 31
    Jan
  • A Comparison of Acquired Port-wine Stain: MATERIALS AND METHODS

Materials

The skin biopsies of 31 patients before treatment were included in this study. The patients consisted of 14 APWS and 17 CPWS. The mean age was 26.7 years in APWS and 25.7 years in CPWS. In APWS, the ages of the onset were from 2 to 40. Con­firmation of the acquired nature of the lesion was obtained in each case by reviewing photographs of the patient taken prior to onset of the lesion. If not available, then family members confirmed the time of onset. The details of these patients are shown in Table 1.

Methods

1) Histopathologic assessment
Punch biopsies taken using local anesthesia were fixed with a formalin-mucriculide-acetic acid (FMA) solution embedded in paraffin. Immunohistochemi- cal stain using factor VIII-related antigen was ob­tained from the biopsy specimens to better visualize the vessels. Detailed histopathologic analyses of each specimen were made with the assistance of a computer assisted image analyzer (Image & Micros­cope Technology™, USA). Using this program, variable parameters of selected objects from the scanned image of the histologic slide were measured automatically. Vessel area, percentage of vascular area (the percentage of dermis occupied by vessels) and vessel depth were measured down to a depth of 1 mm from the dermo-epidermal junction in all slides. For the purposes of analysis, we regarded measured area from the scanned vessel image as actual vessel area. The percentage of vascular area refers to the percentage of summation of vessels over dermal area. The statistical analysis was performed with an SPSS 10.0 statistical program. The Mann- Whitney test was used to compare differences between APWS and CPWS and a p-value of less than 0.05 was considered to be significant.
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Table 1. Summary of patient details including mean data


No.


Sex


Age (yrs)


Age onset (yrs)


Site



Vessel area
(U m2)


%
vascular area (%)



Vessel depth
(U m)


APWS


1


M


14


12


Leg


2806.9


4.77


312.4


2


M


19


18


Forearm


1518.5


1.95


175.3


3


M


29


14


Cheek


928.0


2.52


401.1


4


M


39


18


Cheek


495.1


0.94


289.8


5


F


44


40


Chin


460.4


1.83


393.6


6


F


27


16


Nose


1037.2


1.39


314.8


7


F


14


2


Eyelid


989.2


1.95


364.6


8


F


13


6


Neck


2426.1


4.77


163.7


9


F


21


14


Chin


754.7


0.93


144.1


10


F


44


33


Forehead


255.8


0.62


490.5


11


F


35


29


Eyebrow


359.6


1.33


502.3


12


F


30


30


Forehead


1276.4


2.19


464.8


13


F


22


4


Arm


708.0


3.00


203.1


14


F


15


14


Lower leg


189.3


0.23


364.3


Mean ± SD



1,014.7
±
782.5


2.03
±
1.38



327.5
±
120.7


CPWS


15


M


18


Chin


1424.8


1.88


334.1


16


M


17


Arm


1226.0


2.44


204.7


17


M


29


Cheek


1034.9


3.43


366.9


18


M


38


Face


2869.2


4.18


452.4


19


M


18


Arm


560.5


0.74


339.8


20


F


20


Lip


1002.9


3.52


217.7


21


F


13


Leg


836.1


3.87


177.2


22


F


30


Leg


2016.8


2.30


342.4


23


F


20


Cheek


1188.3


1.62


444.0


24


F


47


Cheek


1360.8


2.84


412.8


25


F


41


Temple


2403.1


3.58


421.1


26


F


29


Leg


1557.0


2.26


414.5


27


F


21


Neck


909.3


1.99


268.0


28


F


35


Cheek


2281.4


7.07


366.3


29


F


16


Leg


1111.2


1.41


258.8


30


F


20


Leg


383.9


0.66


180.8


31


F


25


Arm


639.3


1.30


267.7

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